RW 1 ea0287532-rw_amesite.htm WITHDRAWAL REQUEST

April 23, 2026

Via EDGAR Transmission

Division of Corporation Finance

United States Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549

Re:

Amesite Inc.

Withdrawal of Registration Statement on Form S-1

File No. 333-295187

Ladies and Gentlemen:

Pursuant to Rule 477 under the Securities Act of 1933, as amended (the “Securities Act”), Amesite Inc. (the “Registrant”) hereby requests the immediate withdrawal of the above-referenced registration statement, together with all exhibits and amendments thereto (the “Registration Statement”). The Registration Statement was initially filed with the Securities and Exchange Commission (the “SEC”) on April 20, 2026.

The Registrant is requesting to withdraw the Registration Statement because it has elected not to pursue the sale of securities pursuant to the Registration Statement at this time. The Registrant confirms that no securities were sold in connection with the offering contemplated by the Registration Statement.

Please direct any questions or comments regarding this correspondence to our counsel, Sean F. Reid of Sheppard Mullin Richter & Hampton LLP, at (212) 896-0610.

Thank you for your assistance in this matter.

	Very truly yours,

	AMESITE INC.

	/s/ Ann Marie Sastry
	Names:		Ann Marie Sastry, Ph.D
	Title:		Chief Executive Officer

SEC Form RW filed by Amesite Inc.