SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13D

Under the Securities Exchange Act of 1934

(Amendment No. 7)

Tenaya Therapeutics, Inc.

(Name of Issuer)

Common Stock, par value $0.0001 per share

(Title of Class of Securities)

87990A106

(CUSIP Number)

James Evangelista
1 Letterman Drive, Building D, Suite DM-900
San Francisco, CA, 94129
(415) 865-2050

(Name, Address and Telephone Number of Person Authorized to Receive Notices and Communications)

02/25/2026

(Date of Event Which Requires Filing of This Statement)

If the filing person has previously filed a statement on Schedule 13G to report the acquisition that is the subject of this Schedule 13D, and is filing this schedule because of §§ 240.13d-1(e), 240.13d-1(f) or 240.13d-1(g), check the following box. Checkbox not checked

The information required on the remainder of this cover page shall not be deemed to be “filed” for the purpose of Section 18 of the Securities Exchange Act of 1934 (“Act”) or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

The Column Group III, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 0.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 0.00

Aggregate amount beneficially owned by each reporting person

0.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

0 %

Type of Reporting Person (See Instructions)

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

The Column Group III-A, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 0.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 0.00

Aggregate amount beneficially owned by each reporting person

0.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

0 %

Type of Reporting Person (See Instructions)

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

The Column Group III GP, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 0.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 0.00

Aggregate amount beneficially owned by each reporting person

0.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

0 %

Type of Reporting Person (See Instructions)

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

The Column Group Opportunity III, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 49,313,559.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 49,313,559.00

Aggregate amount beneficially owned by each reporting person

49,313,559.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

23.1 %

Type of Reporting Person (See Instructions)

Comment for Type of Reporting Person:

All such shares are held of record by TCG Opportunity III LP (as defined in Item 2(a) of the Original Schedule 13D). TCG Opportunity III GP LP (as defined in Item 2(a) of the Original Schedule 13D) is the general partner of TCG Opportunity III LP and may be deemed to have voting, investment and dispositive power with respect to these securities. TCG Opportunity III GP LLC (as defined in Item 2(a) of the Original Schedule 13D) is the general partner of TCG Opportunity III GP LP and the ultimate general partner of TCG Opportunity III LP and may be deemed to have voting, investment and dispositive power with respect to these securities. Peter Svennilson and Tim Kutzkey are the managing members of TCG Opportunity III GP LLC and may each be deemed to share voting, investment and dispositive power with respect to these securities. Excludes warrants to purchase 53,571,426 shares of Common Stock held by TCG Opportunity III LP which are not exercisable within 60 days of this Statement due to the Beneficial Ownership Limitations (as defined in Item 6). Based on 213,744,535 shares of Common Stock outstanding as of December 15, 2025, as reported by the Issuer in its prospectus supplement filed pursuant to Rule 424(b)(5) with the Securities and Exchange Commission (the Commission) on December 12, 2025 (the Prospectus).

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

The Column Group Opportunity III GP, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 49,313,559.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 49,313,559.00

Aggregate amount beneficially owned by each reporting person

49,313,559.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

23.1 %

Type of Reporting Person (See Instructions)

Comment for Type of Reporting Person:

All such shares are held of record by TCG Opportunity III LP. TCG Opportunity III GP LP is the general partner of TCG Opportunity III LP and may be deemed to have voting, investment and dispositive power with respect to these securities. TCG Opportunity III GP LLC is the general partner of TCG Opportunity III GP LP and the ultimate general partner of TCG Opportunity III LP and may be deemed to have voting, investment and dispositive power with respect to these securities. Peter Svennilson and Tim Kutzkey are the managing members of TCG Opportunity III GP LLC and may each be deemed to share voting, investment and dispositive power with respect to these securities. Excludes warrants to purchase 53,571,426 shares of Common Stock held by TCG Opportunity III LP which are not exercisable within 60 days of this Statement due to the Beneficial Ownership Limitations. Based on 213,744,535 shares of Common Stock outstanding as of December 15, 2025, as reported by the Issuer in the Prospectus.

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

TCG Opportunity III GP, LLC

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 49,313,559.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 49,313,559.00

Aggregate amount beneficially owned by each reporting person

49,313,559.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

23.1 %

Type of Reporting Person (See Instructions)

Comment for Type of Reporting Person:

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

Tim Kutzkey

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

UNITED STATES

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 49,313,559.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 49,313,559.00

Aggregate amount beneficially owned by each reporting person

49,313,559.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

23.1 %

Type of Reporting Person (See Instructions)

Comment for Type of Reporting Person:

SCHEDULE 13D

CUSIP No.

87990A106

Name of reporting person

Peter Svennilson

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

SWEDEN

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 49,313,559.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 49,313,559.00

Aggregate amount beneficially owned by each reporting person

49,313,559.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

23.1 %

Type of Reporting Person (See Instructions)

Comment for Type of Reporting Person:

SCHEDULE 13D

Item 1.

Security and Issuer

(a)

Title of Class of Securities:

Common Stock, par value $0.0001 per share

(b)

Name of Issuer:

Tenaya Therapeutics, Inc.

(c)

Address of Issuer's Principal Executive Offices:

171 Oyster Point Boulevard, Suite 500, South San Francisco, CALIFORNIA , 94080.

Item 1 Comment:

This Amendment No. 7 (this Amendment No. 7) supplements and amends the Schedule 13D relating to the Common Stock of the Issuer that was filed with the Commission on December 1, 2022 (the Initial Schedule 13D), as amended by Amendment No. 1 filed with the Commission on February 21, 2023, by Amendment No. 2 filed with the Commission on February 14, 2024 and Amendment No. 3 filed with the Commission on March 7, 2025, Amendment No. 4 filed with the Commission on May 7, 2025, Amendment No. 5 filed with the Commission on December 17, 2025 and Amendment No. 6 filed with the Commission on February 12, 2026 (the Initial Schedule 13D, together with all amendments thereto, the Original Schedule 13D). The securities originally reported on the Initial Schedule 13D were previously reported on a Schedule 13G filed with the Commission on February 11, 2022 (the Schedule 13G). The Schedule 13G was filed pursuant to Rule 13d-1(d) of the Securities Exchange Act. Capitalized terms contained herein but not otherwise defined shall have the meanings ascribed to such terms in the Original Schedule 13D.

Item 2.

Identity and Background

(a)

This Statement is being filed by The Column Group III, LP (TCG III LP), The Column Group III-A, LP (TCG III-A LP), The Column Group III GP, LP (TCG III GP LP), The Column Group Opportunity III, LP (TCG Opportunity III LP), The Column Group Opportunity III GP, LP (TCG Opportunity III GP LP), TCG Opportunity III GP, LLC (TCG Opportunity III GP LLC, together with TCG III LP, TCG III-A LP, TCG III GP LP, TCG Opportunity III LP, and TCG Opportunity III GP LP, the Reporting Entities), Tim Kutzkey and Peter Svennilson. The Reporting Entities, Tim Kutzkey and Peter Svennilson are collectively referred to as the Reporting Persons. Tim Kutzkey and Peter Svennilson have been added to this Statement as a Reporting Person as a result of the retirement of a managing partner of TCG III GP LP and a managing member of TCG Opportunity III GP LLC. The agreement among the Reporting Persons to file jointly in accordance with the provisions of Rule 13d-1(k)(1) under the Act is attached as Exhibit 1 to Amendment No. 6. Each Reporting Person disclaims beneficial ownership of all securities reported in this Statement except to the extent of such Reporting Person's pecuniary interest therein, other than those securities reported herein as being held directly by such Reporting Person.

(b)

The address of the principal offices of each Reporting Person is 1 Letterman Drive, Building D, Suite M-900, San Francisco, CA 94129.

(c)

Each of TCG III LP, TCG III-A LP and TCG Opportunity III LP is a venture capital investment entity. TCG III GP LP is the general partner of each of TCG III LP and TCG III-A LP. TCG Opportunity III GP LP is the general partner of TCG Opportunity III LP. TCG Opportunity III GP LLC is the general partner of TCG Opportunity III GP LP and the ultimate general partner of TCG Opportunity III LP. Dr. Kutzkey and Peter Svennilson are the managing partners of TCG III GP LP and the managing members of TCG Opportunity III GP LLC, and are each engaged through venture capital investment entities in acquiring, holding and disposing of interests in various companies for investment purposes.

(d)

During the past five years, none of the Reporting Persons have been convicted in a criminal proceeding (excluding traffic violations or similar misdemeanors).

(e)

None of the Reporting Persons are, nor during the last five years have been, a party to a civil proceeding of a judicial or administrative body of competent jurisdiction and as a result of such proceeding was or is subject to a judgment, decree or final order enjoining future violations of, or prohibiting or mandating activities subject to, federal or state securities laws or finding any violation with respect to such laws.

(f)

Each of TCG III GP LP, TCG III LP, TCG III-A LP, TCG Opportunity III LP and TCG Opportunity III GP LP is a limited partnership organized under the laws of the State of Delaware. TCG Opportunity III GP LLC is a limited company organized under the laws of the State of Delaware. Dr. Kutzkey is a citizen of the United States of America. Mr. Svennilson is a citizen of Sweden.

Item 3.

Source and Amount of Funds or Other Consideration

Item 3 of the Original Schedule 13D is hereby amended by adding the following as the last paragraphs thereof: On February 20, 2026, TCG III LP sold an aggregate of 245,965 shares of the Issuer's Common Stock at a weighted-average price of $0.6149 per share and an aggregate sale price of approximately $0.15 million. On February 20, 2026, TCG III-A LP sold an aggregate of 277,769 shares of the Issuer's Common Stock at a weighted-average price of $0.6149 per share and an aggregate sale price of approximately $0.17 million. On February 23, 2026, TCG III LP sold an aggregate of 143,709 shares of the Issuer's Common Stock at a weighted-average price of $0.5972 per share and an aggregate sale price of approximately $0.09 million. On February 23, 2026, TCG III-A LP sold an aggregate of 162,291 shares of the Issuer's Common Stock at a weighted-average price of $0.5972 per share and an aggregate sale price of approximately $0.1 million. On February 24, 2026, TCG III LP sold an aggregate of 309,228 shares of the Issuer's Common Stock at a weighted-average price of $0.6153 per share and an aggregate sale price of approximately $0.19 million. On February 24, 2026, TCG III-A LP sold an aggregate of 349,212 shares of the Issuer's Common Stock at a weighted-average price of $0.6153 per share and an aggregate sale price of approximately $0.21 million. On February 25, 2026, TCG III LP sold an aggregate of 1,649,281 shares of the Issuer's Common Stock at a weighted-average price of $0.5624 per share and an aggregate sale price of approximately $0.93 million. On February 25, 2026, TCG III-A LP sold an aggregate of 1,862,545 shares of the Issuer's Common Stock at a weighted-average price of $0.5624 per share and an aggregate sale price of approximately $1.05 million.

Item 4.

Purpose of Transaction

The information set forth in Item 4 of the Original Schedule 13D is incorporated herein by reference.

Item 5.

Interest in Securities of the Issuer

(a)

See Items 7-11 of the cover pages of this Amendment No. 7.

(b)

See Items 7-11 of the cover pages of this Amendment No. 7.

(c)

Except as reported in this Statement and in Item 3 of Amendment No. 6, none of the Reporting Persons has affected any transactions in the Issuer's securities within the past 60 days.

(d)

Under certain circumstances set forth in the limited partnership agreements of each of TCG III LP, TCG III-A LP and TCG Opportunity III LP, the general partner and limited partners of TCG III LP, TCG III-A LP and TCG Opportunity III LP, as applicable, may be deemed to have the right to receive dividends from, or the proceeds from, the sale of shares of the Issuer owned by such entity of which they are a partner.

(e)

Not applicable.

Item 6.

Contracts, Arrangements, Understandings or Relationships With Respect to Securities of the Issuer

The information set forth in Item 6 of the Original Schedule 13D is incorporated herein by reference.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

The Column Group III, LP

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

The Column Group III-A, LP

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

The Column Group III GP, LP

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

The Column Group Opportunity III, LP

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

The Column Group Opportunity III GP, LP

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

TCG Opportunity III GP, LLC

Signature:	/s/ James Evangelista, Attorney in Fact
Name/Title:	James Evangelista, Attorney in Fact
Date:	02/26/2026

Tim Kutzkey

Signature:	/s/ James Evangelista
Name/Title:	James Evangelista, Attorney-in-Fact
Date:	02/26/2026

Peter Svennilson

Signature:	/s/ James Evangelista
Name/Title:	James Evangelista, Attorney-in-Fact
Date:	02/26/2026

Amendment: SEC Form SCHEDULE 13D/A filed by Tenaya Therapeutics Inc.