SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13D

Under the Securities Exchange Act of 1934

(Amendment No. 6)

ORCHESTRA BIOMED HOLDINGS, INC.

(Name of Issuer)

Common Stock, par value $0.0001 per share

(Title of Class of Securities)

68572M106

(CUSIP Number)

Roderick Wong, M.D.
RTW Investments, LP, 40 10th Avenue, Floor 7
New York, NY, 10014
(646) 597-6980

(Name, Address and Telephone Number of Person Authorized to Receive Notices and Communications)

02/23/2026

(Date of Event Which Requires Filing of This Statement)

If the filing person has previously filed a statement on Schedule 13G to report the acquisition that is the subject of this Schedule 13D, and is filing this schedule because of §§ 240.13d-1(e), 240.13d-1(f) or 240.13d-1(g), check the following box. Checkbox not checked

The information required on the remainder of this cover page shall not be deemed to be “filed” for the purpose of Section 18 of the Securities Exchange Act of 1934 (“Act”) or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

SCHEDULE 13D

CUSIP No.

68572M106

Name of reporting person

RTW Investments, LP

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

DELAWARE

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 11,942,426.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 11,942,426.00

Aggregate amount beneficially owned by each reporting person

11,942,426.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

19.7 %

Type of Reporting Person (See Instructions)

IA, PN

SCHEDULE 13D

CUSIP No.

68572M106

Name of reporting person

RTW Master Fund, Ltd.

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

CAYMAN ISLANDS

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 5,820,930.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 5,820,930.00

Aggregate amount beneficially owned by each reporting person

5,820,930.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

10.0 %

Type of Reporting Person (See Instructions)

SCHEDULE 13D

CUSIP No.

68572M106

Name of reporting person

RTW Innovation Master Fund, Ltd.

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

CAYMAN ISLANDS

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 4,931,727.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 4,931,727.00

Aggregate amount beneficially owned by each reporting person

4,931,727.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

8.4 %

Type of Reporting Person (See Instructions)

SCHEDULE 13D

CUSIP No.

68572M106

Name of reporting person

Roderick Wong

Check the appropriate box if a member of a Group (See Instructions)

(a)

(b)

SEC use only

Source of funds (See Instructions)

Check if disclosure of legal proceedings is required pursuant to Items 2(d) or 2(e)

Checkbox not checked

Citizenship or place of organization

UNITED STATES

Number of Shares Beneficially Owned by Each Reporting Person With:

7	Sole Voting Power 0.00
8	Shared Voting Power 11,942,426.00
9	Sole Dispositive Power 0.00
10	Shared Dispositive Power 11,942,426.00

Aggregate amount beneficially owned by each reporting person

11,942,426.00

Check if the aggregate amount in Row (11) excludes certain shares (See Instructions)

Checkbox not checked

Percent of class represented by amount in Row (11)

19.7 %

Type of Reporting Person (See Instructions)

HC, IN

SCHEDULE 13D

Item 1.

Security and Issuer

(a)

Title of Class of Securities:

Common Stock, par value $0.0001 per share

(b)

Name of Issuer:

ORCHESTRA BIOMED HOLDINGS, INC.

(c)

Address of Issuer's Principal Executive Offices:

150 Union Square Drive, New Hope, PENNSYLVANIA , 18938.

Item 4.

Purpose of Transaction

Item 4 of the Schedule 13D is hereby amended and supplemented as follows: On February 23, 2026, the RTW Funds submitted notices to the Issuer increasing the Maximum Percentage under the Pre-Funded Warrants from 9.99% to 19.99%. In accordance with the terms of the Pre-Funded Warrants, this increase in the Maximum Percentage will become effective on the 61st day following the delivery of the notice. As a result of the increase in the Maximum Percentage, the Reporting Persons are deemed, effective February 24, 2026, to beneficially own all of the shares underlying the Pre-Funded Warrants, resulting in an increase in the Reporting Persons' aggregate beneficial ownership by 3,636,363 shares of Common Stock. In accordance with the terms of the Pre-Funded Warrants, the RTW Funds reserve the right to decrease the Maximum Percentage, from time to time in the future, to any percentage by written notice to the Issuer.

Item 5.

Interest in Securities of the Issuer

(a)

Items 5(a)-(c) of the Schedule 13D are hereby amended and supplemented as follows: RTW Master Fund is the record owner of 4,394,985 shares of Common Stock and 1,425,945 shares of Common Stock issuable upon exercise of pre-funded warrants ("Pre-Funded Warrants"). RTW Innovation is the record owner of 3,053,082 shares of Common Stock and 1,878,645 shares of Common Stock issuable upon exercise of Pre-Funded Warrants. RTW Biotech Opportunities Operating Ltd ("RTW Biotech Fund") is the record owner of 857,996 shares of Common Stock and 331,773 shares of Common Stock issuable upon exercise of Pre-Funded Warrants. The Pre-Funded Warrants are immediately exercisable in full as of the date hereof. As the investment manager of the funds, RTW Investments may be deemed to own beneficially the shares of Common Stock held by the funds. As the Managing Partner of RTW Investments, Dr. Wong may be deemed to own beneficially the shares of Common Stock. The percentage of outstanding shares of Common Stock of the Issuer which may be deemed to be beneficially owned by each Reporting Person is set forth on Line 13 of such Reporting Person's cover sheet. Such percentage was calculated based on 57,069,462 outstanding shares of Common Stock as of February 19, 2026, as reported to the Reporting Persons by the Issuer.

(b)

See rows (7) through (10) of the cover pages to this Schedule 13D for the number of shares of Common Stock as to which each Reporting Person has the sole or shared power to vote or direct the vote and sole or shared power to dispose or to direct the disposition

(c)

Except as set forth herein, none of the Reporting Persons has effected any transactions with respect to the securities of the Issuer during the past sixty days.

Item 6.

Contracts, Arrangements, Understandings or Relationships With Respect to Securities of the Issuer

The information set forth in Item 4 is incorporated herein by reference.

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

RTW Investments, LP

Signature:	/s/ Roderick Wong, M.D.
Name/Title:	Roderick Wong, M.D., Managing Partner
Date:	02/25/2026

RTW Master Fund, Ltd.

Signature:	/s/ Darshan Patel
Name/Title:	Darshan Patel, Director
Date:	02/25/2026

RTW Innovation Master Fund, Ltd.

Signature:	/s/ Darshan Patel
Name/Title:	Darshan Patel, Director
Date:	02/25/2026

Roderick Wong

Signature:	/s/ Roderick Wong, M.D.
Name/Title:	Roderick Wong, M.D.
Date:	02/25/2026

Amendment: SEC Form SCHEDULE 13D/A filed by Orchestra BioMed Holdings Inc.